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Toxicity of Glutathione-Binding Metals: A Review of Targets and Mechanisms

Addgene) via Golden Gate assembly

In this context, buthionine sulfoximine (BSO) is the most popular GSH-depleting agent studied in both preclinical and early clinical trials, but limitation on its availability has led to a search for alternatives [13, 14]

In short, sgRNA targeting the first intron of MT1E (sgMT1E_Intron GAAAGCATCTAACGAAGTAC) was designed with Benchling, and corresponding forward and reverse oligos, flanked by BbsI-cutting sites, were annealed and cloned into pX330-Cas9 (#42230

Some individuals may experience mild side effects such as redness or soreness at the injection site

Medical supervision: not required